Yet another lawsuit has been filed against Elon Musk's Twitter, this time from women employees accusing the company of gender discrimination in recent mass layoffs.

Twitter began the year with 7,500 employees, before laying off around 3,700 employees in early November, at the urging of owner Elon Musk, which prompted several hundred others to resign.

The lawsuit from two women employees, filed late on Wednesday in San Francisco, claims that since Musk's acquisition of the social media platform, 57 percent of the company's women have been laid off, as opposed to 47 percent of the men. The disparity was worse among engineers, where 63 percent of women were fired compared to 48 percent of men.

Elon Musk has previously been sued by women at his SpaceX company, who accused HR of ignoring sexual harassment complaints. They also claimed Musk's media persona and online rhetoric encouraged a toxic work culture degrading to women.

In addition to the lawsuit from women, three other lawsuits have been filed against Twitter this year for failing to provide the advanced notice for terminations required by law, refusing to pay severance packages, and icing out disabled workers by not allowing them to work remotely.

While the company has denied wrongdoing in all cases, the women in the most recent lawsuit accuse the company of violating federal and California laws which ban workplace sex discrimination.

Shannon Liss-Riordan, a lawyer for the plaintiffs, told Reuters that "regardless of their talent and contributions," once Musk took over, women “had targets on their backs."

“The mass termination of employees at Twitter has impacted female employees to a much greater extent than male employees – and to a highly statistically significant degree,” Liss-Riordan wrote. “Moreover, Elon Musk has made a number of publicly discriminatory remarks about women, further confirming that the mass termination’s greater impact on female employees resulted from discrimination.”